The glycocalyx affects the mechanotransductive perception of the topographical microenvironment
Articolo
Data di Pubblicazione:
2022
Citazione:
The glycocalyx affects the mechanotransductive perception of the topographical microenvironment / M. Chighizola, T. Dini, S. Marcotti, M. D’Urso, C. Piazzoni, F. Borghi, A. Previdi, L. Ceriani, C. Folliero, B. Stramer, C. Lenardi, P. Milani, A. Podestà, C. Schulte. - In: JOURNAL OF NANOBIOTECHNOLOGY. - ISSN 1477-3155. - 20:1(2022), pp. 418.1-418.20. [10.1186/s12951-022-01585-5]
Abstract:
The cell/microenvironment interface is the starting point of integrin-mediated mechanotransduction, but many
details of mechanotransductive signal integration remain elusive due to the complexity of the involved (extra)cellular
structures, such as the glycocalyx. We used nano-bio-interfaces reproducing the complex nanotopographical features
of the extracellular matrix to analyse the glycocalyx impact on PC12 cell mechanosensing at the nanoscale (e.g., by
force spectroscopy with functionalised probes). Our data demonstrates that the glycocalyx configuration affects
spatio-temporal nanotopography-sensitive mechanotransductive events at the cell/microenvironment interface.
Opposing effects of major glycocalyx removal were observed, when comparing flat and specific nanotopographical
conditions. The excessive retrograde actin flow speed and force loading are strongly reduced on certain nanotopog-
raphies upon strong reduction of the native glycocalyx, while on the flat substrate we observe the opposite trend.
Our results highlight the importance of the glycocalyx configuration in a molecular clutch force loading-dependent
cellular mechanism for mechanosensing of microenvironmental nanotopographical features.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Adhesion force spectroscopy; Atomic force microscopy; Colloidal probes; Focal adhesion; Force loading; Glycocalyx; Integrin adhesion complexes; Mechanotransduction; Molecular clutch; Nanostructured cell microenvironment; Nanotopography
Elenco autori:
M. Chighizola, T. Dini, S. Marcotti, M. D’Urso, C. Piazzoni, F. Borghi, A. Previdi, L. Ceriani, C. Folliero, B. Stramer, C. Lenardi, P. Milani, A. Podestà, C. Schulte
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