Carbohydrate-carbohydrate interaction drives the preferential insertion of dirhamnolipid into glycosphingolipid enriched membranes
Articolo
Data di Pubblicazione:
2022
Citazione:
Carbohydrate-carbohydrate interaction drives the preferential insertion of dirhamnolipid into glycosphingolipid enriched membranes / V. Rondelli, L. Mollica, A. Koutsioubas, N. Nasir, M. Trapp, E. Deboever, P. Brocca, M. Deleu. - In: JOURNAL OF COLLOID AND INTERFACE SCIENCE. - ISSN 0021-9797. - 616:(2022 Jun), pp. 739-748. [10.1016/j.jcis.2022.02.120]
Abstract:
Rhamnolipids (RLs) are among the most important biosurfactants produced by microorganisms, and have been widely investigated because of their multiple biological activities. Their action appears to depend on their structural interference with lipid membranes, therefore several studies have been performed to investigate this aspect. We studied by X-ray scattering, neutron reflectometry and molecular dynamic simulations the insertion of dirhamnolipid (diRL), the most abundant RL, in model cellular membranes made of phospholipids and glycosphingolipids. In our model systems the affinity of diRL to the membrane is highly promoted by the presence of the glycosphingolipids and molecular dynamics simulations unveil that this evidence is related to sugar-sugar attractive interactions at the membrane surface. Our results improve the understanding of the plethora of activities associated with RLs, also opening new perspectives in their selective use for pharmaceutical and cosmetics formulations. Additionally, they shed light on the still debated role of carbohydrate-carbohydrate interactions as driving force for molecular contacts at membrane surface.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
GM1 ganglioside; MD simulations; model membrane; neutron reflectometry; SAXS
Elenco autori:
V. Rondelli, L. Mollica, A. Koutsioubas, N. Nasir, M. Trapp, E. Deboever, P. Brocca, M. Deleu
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