Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection
Articolo
Data di Pubblicazione:
2009
Citazione:
Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection / R.C. Paroni, E.M. Faioni, C. Razzari, G. Fontana, M.N. Cattaneo. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 877:3(2009 Jan 15), pp. 351-354. [10.1016/j.jchromb.2008.12.044]
Abstract:
We describe a procedure for quantification of vitamin K-1 in human plasma by HPLC. Samples, enriched with a vitamin K derivative as internal standard, were deproteinized, purified on polymeric RP-SPE cartridges and injected into HPLC equipped with a post-column on-line zinc metal reactor and a fluorometric detector. Median level in blood donors (n = 87) was 1.967 nmol/L(0.93-4.01, 5th-95th percentiles), with a significant correlation between plasma levels and age (r = 0.276, p = 0.00958) and a lower (not significant) value in women than in men. This method, easy-to-handle and with a high throughput, can be used to identify covert states of vitamin K intake deficiency in patients thus at risk of alterations in blood clotting or bone mineralization.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Vitamin K-1; HPLC; Reference values; Haemostasis alteration; Bone mineralization
Elenco autori:
R.C. Paroni, E.M. Faioni, C. Razzari, G. Fontana, M.N. Cattaneo
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