Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping
Academic Article
Publication Date:
2003
Citation:
Detection of HLA polymorphisms by ligase detection reaction and a universal array format: a pilot study for low resolution genotyping / C. Consolandi, E. Busti, C. Pera, L. Delfino, G.B. Ferrara, R. Bordoni, B. Castiglioni, L. Rossi Bernardi, C. Battaglia, G. De Bellis. - In: HUMAN IMMUNOLOGY. - ISSN 0198-8859. - 64:1(2003 Jan), pp. 168-178.
abstract:
We present our results in the identification of polymorphic sites within
the second exon of the human leukocyte antigen A (HLA-A) region using the
DNA microarray technology. Allele specific detection was performed by
polymerase chain reaction followed by ligase detection reaction (LDR) in
combination with a universal array, a powerful method for high throughput
DNA sequence analysis. By this approach we confirmed 32 human samples
previously characterized by direct DNA sequencing, thus demonstrating the
interest of this approach.
the second exon of the human leukocyte antigen A (HLA-A) region using the
DNA microarray technology. Allele specific detection was performed by
polymerase chain reaction followed by ligase detection reaction (LDR) in
combination with a universal array, a powerful method for high throughput
DNA sequence analysis. By this approach we confirmed 32 human samples
previously characterized by direct DNA sequencing, thus demonstrating the
interest of this approach.
IRIS type:
01 - Articolo su periodico
Keywords:
HLA-A Antigenes genetics, Oligonucleotide Array sequence analysis methods, polymorphism genetic
List of contributors:
C. Consolandi, E. Busti, C. Pera, L. Delfino, G.B. Ferrara, R. Bordoni, B. Castiglioni, L. Rossi Bernardi, C. Battaglia, G. De Bellis
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