Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR
Academic Article
Publication Date:
2001
Citation:
Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR / E. Evron, W.C. Dooley, C.B. Umbricht, D. Rosenthal, N. Sacchi, E. Gabrielson, A.B. Soito, D.T. Hung, B. Ljung, N.E. Davidson, S. Sukumar. - In: THE LANCET. - ISSN 0140-6736. - 357:9265(2001 Apr 28), pp. 1335-1336. [10.1016/S0140-6736(00)04501-3]
abstract:
If detected early, breast cancer is curable. We tested cells collected from the breast ducts by methylation-specific PCR (MSP). Methylated alleles of Cyclin D2, RAR-beta, and Twist genes were frequently detected in fluid from mammary ducts containing endoscopically visualised carcinomas (17 cases of 20), and ductal carcinoma in situ (two of seven), but rarely in ductal lavage fluid from healthy ducts (five of 45). Two of the women with healthy mammograms whose ductal lavage fluid contained methylated markers and cytologically abnormal cells were subsequently diagnosed with breast cancer. Carrying out MSP in these fluid samples may provide a sensitive and powerful addition to mammographic screening for early detection of breast cancer.
IRIS type:
01 - Articolo su periodico
Keywords:
sensitivity and specificity ; polymerase chain reaction ; promoter regions, genetic ; therapeutic irrigation ; DNA methylation ; humans ; breast ; breast neoplasms ; female
List of contributors:
E. Evron, W.C. Dooley, C.B. Umbricht, D. Rosenthal, N. Sacchi, E. Gabrielson, A.B. Soito, D.T. Hung, B. Ljung, N.E. Davidson, S. Sukumar
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