Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy
Academic Article
Publication Date:
2010
Citation:
Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy / F. Colleoni, D. Lattuada, A. Garretto, M. Massari, C. Mandò, E. Somigliana, I. Cetin. - In: AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY. - ISSN 0002-9378. - 203:4(2010), pp. 365.e1-365.e6. [10.1016/j.ajog.2010.05.027]
abstract:
OBJECTIVE: We investigated mitochondrial DNA (mtDNA) content in the maternal circulation of normal pregnancies of different gestational ages and in pregnancies complicated by intrauterine growth restriction (IUGR).
STUDY DESIGN: We examined 70 maternal blood samples: 13 nonpregnant women; 45 normal pregnancies, divided into the 3 trimesters;
and 12 pregnancies complicated by IUGR. MtDNA content was determined by real-time quantitative polymerase chain reaction, using a genomic control and a target gene.
RESULTS: A highly significant progressive reduction in circulating mtDNA was observed in pregnant women of first, second, and third trimesters and compared to nonpregnant women (mean value: 237, 188, 144, and 283, respectively; P .001). Moreover, mtDNA was significantly increased in women carrying IUGR fetuses compared to women with normal pregnancies (430 vs 144; P .001).
CONCLUSION: MtDNA could provide new insight into the mechanisms that occur during physiological gestation. Furthermore, mtDNA content may help recognize the IUGR disease in pregnancy.
STUDY DESIGN: We examined 70 maternal blood samples: 13 nonpregnant women; 45 normal pregnancies, divided into the 3 trimesters;
and 12 pregnancies complicated by IUGR. MtDNA content was determined by real-time quantitative polymerase chain reaction, using a genomic control and a target gene.
RESULTS: A highly significant progressive reduction in circulating mtDNA was observed in pregnant women of first, second, and third trimesters and compared to nonpregnant women (mean value: 237, 188, 144, and 283, respectively; P .001). Moreover, mtDNA was significantly increased in women carrying IUGR fetuses compared to women with normal pregnancies (430 vs 144; P .001).
CONCLUSION: MtDNA could provide new insight into the mechanisms that occur during physiological gestation. Furthermore, mtDNA content may help recognize the IUGR disease in pregnancy.
IRIS type:
01 - Articolo su periodico
Keywords:
Intrauterine growth restriction ; maternal blood ; mitochondrial DNA ; pregnancy :
List of contributors:
F. Colleoni, D. Lattuada, A. Garretto, M. Massari, C. Mandò, E. Somigliana, I. Cetin
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