Persona
immagine segnaposto

BESUSSO DARIO

Ricercatore a tempo determinato in Tenure Track
Dipartimento di Bioscienze

Pubblicazioni (30)

Network di ricerca (2)

NeuroStemCellReconstruct - NSC-Reconstruct project aims at repairing the damaged brain cells and reconstructing our brain circuits using neurons and reprogrammed cells, derived from stem cells. This type of cell-based strategy constitutes a very promising approach for the treatment of those neurodegenerative diseases where only a single and confined subtype of cells is damaged. This is the case of Parkinson’s disease, where promising preliminary results have been obtained using fetal cells for transplantation (Barker, 2015). Huntington’s disease is another example where the replacement of a single cell type is needed, and for which fetal tissue transplantations have been conducted and stem cell based therapies are actively being developed (Precious, 2017). European networks involving several partners from this consortium have been pioneers in the development of stem cell-based therapies for PD and HD, and are leading a European pluripotent stem cell clinical trial for PD projected to start in 2020 (Barker, 2018). (01/04/2019 - 31/12/2024)
NeuroStemCellRepair - Neurostemcellrepair aims at taking human stem cells through the final steps toward clinical application in cell replacement therapy for neurological disorders. PD will be taken as the prototypical disease because stem cell therapy is now close to clinical translation. Moreover, we will tackle next generation issues pertaining to stem cells at a basic level and develop new approaches and novel cell sources, validated at pre-clinical stages, for both PD and HD. The project teams represent a wide range of competences, including stem cell specialists, developmental neurobiologists, experts in neurodegeneration, scientists with links to the clinic and stem cell manufacturing/clinical validation. The research plan is constructed on exchange of tools, sharing of protocols and expertise and joint deliverables among the participants. We will address issues related to the control of progenitor proliferation and differentiation into authentic, functional and phenotypically stable dopaminergic or striatal neurons, and exploit new technology for cell reprogramming. We will develop strategies to obtain endurable donor cell engraftment in the host, including acquisition of specific neuronal identities and functional integration in the recipient brain. The therapeutic effect will be evaluated following transplantation in animal models of PD and HD. Cutting edge technologies will be guaranteed by the involvement of three SMEs, one industry and partners experienced in bioengineering, who will collectively provide a toolbox to deliver ontogenetic and reprogramming factors, small molecules and miRNA, immunoseparation strategies, in vivo monitoring of donor cell behaviour, scale up and GMP-compliant protocols. Ultimately, Neurostemcellrepair is expected to develop new cell sources based on cellular reprogramming, make significant advance towards stem cell therapy in HD, and close the gap between development and clinical implementation of stem cell replacement therapies for PD. (01/04/2014 - 31/03/2024)
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