Urinary peptidome and proteome alterations related to tumor progression and invasion in RCC
Abstract
Data di Pubblicazione:
2016
Citazione:
Urinary peptidome and proteome alterations related to tumor progression and invasion in RCC / C. Chinello, M. Grasso, M. Cazzaniga, G. De Sio, A. Grasso, B. Rocco, A. Smith, I. Zoppis, G. Mauri, F. Magni. - In: THE JOURNAL OF UROLOGY. - ISSN 0022-5347. - 195:4 suppl.(2016), pp. e1105-e1105. ((Intervento presentato al convegno Annual Meeting AUA tenutosi a San Diego nel 2016 [10.1016/j.juro.2016.02.2285].
Abstract:
INTRODUCTION AND OBJECTIVES: Up to now, for renal cell
carcinoma (RCC), tumor size and growth rate are the most used
prognostic factors at diagnosis likely being related with potential progressive
neoplasms. Moreover, the degree of the free tumor extension
into renal vessel seems to contribute to disease-free survival and to
influence prognosis. Thus, firstly, in order to highlight urinary peptidome
signatures that might reflect kidney cancer progression, a systematic
investigation by MALDI profiling was carried out identifying endogenous
peptides that show a correlation between their abundance in urine and
pT, tumor size and grade. Secondly, in order to explore proteome
changes reflecting the local tumor infiltration through vena cava, a labelfree
nLC MS/MS was performed in trypsinized urine from subjects
affected by RCC at different level of invasion (vascular endothelium/
vein/ thrombosis).
METHODS: Peptidomic evaluation of tumor progression was
performed in 117 RCC urine samples, through the application of
C8 functionalized magnetic beads purification followed by MALDI-TOF
and statistical analysis for group comparisons and correlations.
nLC-ESI-MS/MS was used for peptide identification. Urine proteome
investigation correlated to different renal vein invasion extent was
carried out by label-free nLC MS/MS strategy using FASP digested
pools of 3 groups. Statistical evaluation was obtained by Progenesis
QI for Proteomics.
RESULTS: 15 endogenous peptides showed a statistically
significant correlation between their urinary concentration and tumor
size (only 3 negatively), 26 with pT (only 6 negatively), and only 5 with
grade (only 1 positively). Most of them were differentially represented in
urine of RCC patients compared to controls and for some of them varied
according to pT or stage. Identity of several of them are likely to confirm
their possible role in tumor progression. Proteomic labelfree approach
regarding tumor invasion allowed to select 671 protein IDs significantly
altered in at least one of the 3 group comparisons (fold change¼2).
12 of them showed a progressive increase from the endothelium wall
infiltration to thrombosis
CONCLUSIONS: These methodological approaches applied to
urine may provide useful keys to highlight alterations triggered by RCC
aggressiveness or vascular neoplastic infiltration from endothelial layer
to vein ostium obstruction, better understand tumor protein handling
aspects, and describe such a dynamic system as growing cancer
cells are.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
C. Chinello, M. Grasso, M. Cazzaniga, G. De Sio, A. Grasso, B. Rocco, A. Smith, I. Zoppis, G. Mauri, F. Magni
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