Performances of cytology and flow cytometry to predict diagnosis of canine lymphoma and subtypes
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Data di Pubblicazione:
2021
Citazione:
Performances of cytology and flow cytometry to predict diagnosis of canine lymphoma and subtypes / S. Comazzi, V. Martini. ((Intervento presentato al convegno ESVCP-ECVCP congress tenutosi a Online nel 2021.
Abstract:
Cytology plays a central role in the diagnostic algorythm for canine lymphoma. Canine lymphoma is a great
candidate to be sampled via fine needle aspiratebiopsy since it is mainly a multicentric disease, involvement of
superficial nodes is frequent and the architectural histological pattern is diffuse in most cases. Accuracy may be
implemented by ancillary techniques such as flow cytometry (FC) and immunocytochemistry (ICC). However, the
diagnostic performances of cytology alone with or without some ancillary techniques is not completely elucidated
in veterinary medicine. In a recent metanalysis in humans, cytology alone showed a sensitivity of 87% and a
specificity of 97% in the identification of lymphoma vs non-lymphoma. This values increased by adding FC or ICC.
However, no data on the performances in identifying specific lymphoma subtypes via cytology were described.
In dogs the current classifications used for canine lymphoma are the updated Kiel and the WHO classifications. The
updated Kiel classification is mainly a cytological scheme based on morphological aspects and it demonstrated a
fair agreement among readers in a previous study. In contrast, WHO is mainly based on histological criteria but
previous papers suggest a possible correlation between these two diagnostic schemes. We recently evaluated
the diagnostic performances and repeatibility of cytology alone for 1) predicting lymphoma vs non-lymphoma; 2)
predicting grade and subtype and 3) predicting WHO subtype.
Cytology showed good performances (sensitivity 92.6%, specificity 89.4%) and repeatibilities in predicting
lymphoma vs non-lymphoma. As expected this values decrease for the evaluation of grade and phenotype, and
for WHO subtype. The major diagnostic challenges were the identification of the grade of B cell lymphomas
and the identification of the immunophenotype for high grade lymphomas with many B cell lymphoma cases
minsinterpreted as T cell.
Flow cytometry may easily help to definitely diagnose the immunophenotype thus solving this issue. On the
other side it may also be useful to discriminate between high and low grade lymphomas by possibly evaluating
the percentage of KI67 positive cells. Further studies on the diagnostic performance of cytology + FC for canine
lymphoma are needed and currently in process.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
S. Comazzi, V. Martini
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