Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis
Articolo
Data di Pubblicazione:
2009
Citazione:
Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis / C. Giuliani, F. Troglio, Z. Bai, F.B. Patel, A. Zucconi1, M.G. Malabarba, A. Disanza, T.B. Stradal, G. Cassata, S. Confalonieri, J.D. Hardin, M.C. Soto, B.D. Grant, G. Scita. - In: PLOS GENETICS. - ISSN 1553-7390. - 5:10(2009 Oct), p. e1000675.e1000675.
Abstract:
The TOCA family of F-BAR–containing proteins bind to and remodel lipid bilayers via their conserved F-BAR domains, and
regulate actin dynamics via their N-Wasp binding SH3 domains. Thus, these proteins are predicted to play a pivotal role in
coordinating membrane traffic with actin dynamics during cell migration and tissue morphogenesis. By combining genetic
analysis in Caenorhabditis elegans with cellular biochemical experiments in mammalian cells, we showed that: i) loss of
CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with
CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect.
Oocyte endocytosis defects correlated well with reduced egg production in these mutants. ii) CeTOCA proteins localize to
cell–cell junctions and are required for proper embryonic morphogenesis, to position hypodermal cells and to organize
junctional actin and the junction-associated protein AJM-1. iii) Double mutant analysis indicated that the toca genes act in
the same pathway as the nematode homologue of N-WASP/WASP, wsp-1. Furthermore, mammalian TOCA-1 and C. elegans
CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. Thus, we propose that TOCA
proteins control tissues morphogenesis by coordinating Clathrin-dependent membrane trafficking with WAVE and NWASP–
dependent actin-dynamics
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
C. Giuliani, F. Troglio, Z. Bai, F.B. Patel, A. Zucconi1, M.G. Malabarba, A. Disanza, T.B. Stradal, G. Cassata, S. Confalonieri, J.D. Hardin, M.C. Soto, B.D. Grant, G. Scita
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