Data di Pubblicazione:
2018
Citazione:
Multiprobe In-Situ hybridization to whole mount arabidopsis seedlings / L. Bruno, F. Araniti, O. Gagliardi - In: Advances in Plant Ecophysiology Techniques / [a cura di] A.M. Sánchez-Moreiras, M.J. Reigosa. - [s.l] : Springer International Publishing, 2018. - ISBN 978-3-319-93232-3. - pp. 295-307 [10.1007/978-3-319-93233-0_18]
Abstract:
In the multicellular organisms to know the temporal and spatial expression of genes gives specific information about the putative sites of activity of the corresponding proteins and also their function related to processes of growth and development as well as in response to biotic and abiotic stress conditions. In-situ RNA-RNA hybridization (ISH) is a powerful technique that enables the localization of gene transcripts at the cellular level by using labeled probes complementary to nucleic acid of interest, followed by probe specific detection. Original methods for ISH have been developed in the 1980s. Since that time, the methodologies associated with it have undergone a continuous evolution, which is also reviewed in the present chapter.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Specific Detection Probes; Wash Samples; Diethylprocarbonate (DEPC); Reagent Setup; Alexa Fluor Dyes
Elenco autori:
L. Bruno, F. Araniti, O. Gagliardi
Link alla scheda completa:
Titolo del libro:
Advances in Plant Ecophysiology Techniques