Transcriptomic analysis of HCN-2 cells suggests connection among oxidative stress, senescence, and neuron death after SARS-CoV-2 infection
Articolo
Data di Pubblicazione:
2021
Citazione:
Transcriptomic analysis of HCN-2 cells suggests connection among oxidative stress, senescence, and neuron death after SARS-CoV-2 infection / A. Valeri, L. Chiricosta, V. Calcaterra, M. Biasin, G. Cappelletti, S. Carelli, G.V. Zuccotti, P. Bramanti, G. Pelizzo, E. Mazzon, A. Gugliandolo. - In: CELLS. - ISSN 2073-4409. - 10:9(2021 Aug 25), pp. 2189.1-2189.15. [10.3390/cells10092189]
Abstract:
According to the neurological symptoms of SARS-CoV-2 infection, it is known that the nervous system is influenced by the virus. We used pediatric human cerebral cortical cell line HCN-2 as a neuronal model of SARS-CoV-2 infection, and, through transcriptomic analysis, our aim was to
evaluate the effect of SARS-CoV-2 in this type of cells. Transcriptome analyses revealed impairment in TXN gene, resulting in deregulation of its antioxidant functions, as well as a decrease in the DNA-repairing mechanism, as indicated by the decrease in KAT5. Western blot analyses of SOD1
and iNOS confirmed the impairment of reduction mechanisms and an increase in oxidative stress. Upregulation of CDKN2A and a decrease in CDK4 and CDK6 point to the blocking of the cell cycle that, according to the deregulation of repairing mechanism, has apoptosis as the outcome. A high
level of proapoptotic gene PMAIP1 is indeed coherent with neuronal death, as also supported by increased levels of caspase 3. The upregulation of cell-cycle-blocking genes and apoptosis suggests a
sufferance state of neurons after SARS-CoV-2 infection, followed by their inevitable death, which
can explain the neurological symptoms reported. Further analyses are required to deeply explain
the mechanisms and find potential treatments to protect neurons from oxidative stress and prevent
their death.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
oxidative stress; ROS homeostasis; cell cycle; apoptosis; HCN-2; SARS-CoV-2
Elenco autori:
A. Valeri, L. Chiricosta, V. Calcaterra, M. Biasin, G. Cappelletti, S. Carelli, G.V. Zuccotti, P. Bramanti, G. Pelizzo, E. Mazzon, A. Gugliandolo
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