Total antioxidant performance: a validated fluorescence assay for the measurement of plasma oxidizability
Articolo
Data di Pubblicazione:
2006
Citazione:
Total antioxidant performance: a validated fluorescence assay for the measurement of plasma oxidizability / G. Beretta, G. Aldini, R. Maffei Facino, R. M. Russell, N. I. Krinsky, K. J. Yeum. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 354:2(2006), pp. 290-298. [10.1016/j.ab.2006.04.001]
Abstract:
The antioxidant capacity of human plasma was determined by following the oxidation kinetics of the lipid-soluble fluorescent marker BODIPY using 2,20-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN) as the lipophilic radical initiator. The results are
expressed as a total antioxidant performance (TAP) value based on the inhibition of BODIPY oxidation, as determined by the appearance
of green fluorescence, with respect to a control sample (phosphatidylcholine with or without delipidized human serum). The suitability
of the assay was evaluated on the basis of its precision, reproducibility, and specificity. The intra- and interassay coefficients of
variation both were less than 5%. The addition of a representative substrate of plasma peroxidation, phosphatidylcholine, up to 750 lg/ml did not induce significant changes in the TAP value. Also, BODIPY photooxidation was not observed during the experimental time course (220 min). The TAP values of 6 plasma samples from healthy donors were measured and correlated with the main plasma waterand
lipid-soluble antioxidants (uric acid and ascorbic acid, a-tocopherol, and carotenoids) and lipid profiles. Significant correlations were
found between TAP and uric acid (R = 0.97, P < 0.05) and cholesterol-adjusted a-tocopherol (R = 0.93, P < 0.01). The results confirm
that the TAP assay is suitable to measure the antioxidant activity of plasma antioxidants localized in both the lipophilic and hydrophilic compartments
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Antioxidant capacity; BODIPY; Human plasma; MeO-AMVN; Oxidative stress; Total antioxidant performance
Elenco autori:
G. Beretta, G. Aldini, R. Maffei Facino, R.M. Russell, N.I. Krinsky, K.J. Yeum
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