Data di Pubblicazione:
2021
Citazione:
In Vitro Silencing of lncRNAs Using LNA GapmeRs / E. Taiana, V. Favasuli, D. Ronchetti, E. Morelli, P. Tassone, G. Viglietto, N.C. Munshi, A. Neri, N. Amodio (METHODS IN MOLECULAR BIOLOGY). - In: Long Non-Coding RNAs in Cancer[s.l] : Springer, 2021. - ISBN 9781071615805. - pp. 157-166 [10.1007/978-1-0716-1581-2_10]
Abstract:
Despite substantial advancements have been achieved in the identification of long noncoding RNA (lncRNA) molecules, many challenges still remain into their functional characterization. Loss-of-function approaches are needed to study oncogenic lncRNAs, which appear more difficult to knock down by RNA interference as compared to mRNAs. In this chapter, we present a protocol based on the use of a novel class of antisense oligonucleotides, named locked nucleic acid (LNA) GapmeRs, to inhibit the oncogenic lncRNA NEAT1 in multiple myeloma cells. Overall, this approach holds many advantages, including its possible independence from delivery reagents as well as the capability to knock down lncRNAs even in hard-to-transfect suspension cells, like hematopoietic cells.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
ncRNA; lncRNA; ASO; LNA-GapmeR; Electroporation; Gymnosis; Multiple myeloma cells
Elenco autori:
E. Taiana, V. Favasuli, D. Ronchetti, E. Morelli, P. Tassone, G. Viglietto, N.C. Munshi, A. Neri, N. Amodio
Link alla scheda completa:
Titolo del libro:
Long Non-Coding RNAs in Cancer