Klebsiella aerogenes and Comamonas testosteroni as bioremoval agents on graffiti-coated concrete and granite : Impact assessment through surface analysis
Articolo
Data di Pubblicazione:
2021
Citazione:
Klebsiella aerogenes and Comamonas testosteroni as bioremoval agents on graffiti-coated concrete and granite : Impact assessment through surface analysis / P. Sanmartín, P. Bosch-Roig, D. Gulotta, R. Fort, I. Bosch, F. Cappitelli. - In: INTERNATIONAL BIODETERIORATION & BIODEGRADATION. - ISSN 0964-8305. - 161:(2021 Jul), pp. 105244.1-105244.9. [10.1016/j.ibiod.2021.105244]
Abstract:
Klebsiella aerogenes ATCC 13048 and Comamonas sp. ATCC 700440 (here identified as Comamonas testosteroni)
have previously been shown to be good candidates for graffiti bioremoval, demonstrating in both immersion and
subaerial strategies high levels of tolerance to the presence of the graffiti paint and some ability to degrade the
graffiti material. To explore further the graffiti bioremoval capacity by these two newly found suitable bacteria,
an experiment was carried out encompassing an improved assay protocol (protocol time was reduced from 20 to
14 days). The formation of pinholes - noticeably higher on concrete than on granite - was already observable by
naked eye and further proved by digital image analysis, novel to this experiment, which showed holes greater in
number due to K. aerogenes and greater in size due to C. testosteroni. Complementarily, surface microtopography -
also novel for bioremoval studies with bacteria - offered detailed information on surface irregularities that allows
better understanding of the performance of the bacteria. In contrast, non-mapping techniques, such as wetting by
droplet, specular gloss and roughness measured in line transects provided less information for the study. Infrared
(ATR-FTIR) spectroscopy and colour change assessment – mainly in the achromatic parameter L* – showed more
intense changes by Comamonas testosteroni.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bacteria; Biocleaning; Graffiti; Microbial degradation; Stone Cultural heritage
Elenco autori:
P. Sanmartín, P. Bosch-Roig, D. Gulotta, R. Fort, I. Bosch, F. Cappitelli
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