Optical Measurements of Sweat for in Vivo Quantification of CFTR Function in Individual Sweat Glands
Articolo
Data di Pubblicazione:
2021
Citazione:
Optical Measurements of Sweat for in Vivo Quantification of CFTR Function in Individual Sweat Glands / D. Treggiari, K. Kleinfelder, M. Bertini, G. Tridello, A. Fedrigo, E. Pintani, P. Iansa, A. Casiraghi, P. Minghetti, M. Cipolli, C. Sorio, P. Melotti. - In: JOURNAL OF CYSTIC FIBROSIS. - ISSN 1569-1993. - 20:5(2021 Sep), pp. 824-827. [10.1016/j.jcf.2021.03.003]
Abstract:
Optical measurement of CFTR-dependent sweat secretion stimulated by a beta-adrenergic cocktail (C-phase) vs. CFTR-independent sweat secretion induced by methacholine (M-phase) can discriminate cystic fibrosis (CF) patientts from controls and healthy carriers by the ratio of sweat rate in the C-phase vs. the M-phase (C/M ratio). However, image analysis is experimentally demanding and time-consuming. Here, sweat droplet number (SDN) in the C-phase, corresponding to the number of sweat-secreting glands, was a statistically significant predictor for detecting the effects of CFTR-targeted therapy. We show that in 44 non-CF subjects and 110 CF patients, SDN in the C-phase provides a linear readout of CFTR function that is more sensitive than that using the C/M ratio. In CF patients, increased SDN in the C-phase during treatment with (LUMA/IVA) was associated with a trend toward improved lung function (FEV1). Our method is suitable for multicenter monitoring of the effects of CFTR modulators.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
CFTR-targeted therapy; cystic fibrosis; image analysis; Sweat glands
Elenco autori:
D. Treggiari, K. Kleinfelder, M. Bertini, G. Tridello, A. Fedrigo, E. Pintani, P. Iansa, A. Casiraghi, P. Minghetti, M. Cipolli, C. Sorio, P. Melotti
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