Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A
Articolo
Data di Pubblicazione:
2009
Citazione:
Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A / S. Cristoni, L. Zingaro, C. Canton, P. Cremonesi, B. Castiglioni, S. Morandi, M. Brasca, M. Luzzana, C. Battaglia. - In: JOURNAL OF MASS SPECTROMETRY. - ISSN 1076-5174. - 44:10(2009), pp. 1482-1488. [10.1002/jms.1636]
Abstract:
Surface-activated chemical ionization (SACI) has been widely used in recent years
for the analysis of different compounds (e.g. peptides, street drugs, amino
acids). The main benefits of this technology are its high sensitivity and its
effectiveness under different chromatographic conditions [i.e. ion exchange
chromatography and reversed-phase (RP) chromatography]. Here we used SACI in
conjunction with quadrupole time-of-flight mass spectrometry to analyze
enterotoxin A, which is produced by Staphylococcus aureus, in milk matrix using
both RP and ion exchange chromatographies. SACI had increased sensitivity as
compared with electrospray ionization. Moreover, the higher quantitation
efficiency of this technique, mainly in terms of limit of detection (0.01 ng/ml),
limit of quantitation (0.05 ng/ml), linearity range (0.05-50 ng/ml), matrix
effect, accuracy (intraday and interday accuracy errors were 9.2% and 10.3%,
respectively) and precision (intraday and interday precision errors were 5.3% and
12.8%, respectively), is shown and discussed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
mass spectrometry ; milk ; staphylococcus ; chromatography
Elenco autori:
S. Cristoni, L. Zingaro, C. Canton, P. Cremonesi, B. Castiglioni, S. Morandi, M. Brasca, M. Luzzana, C. Battaglia
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