HisE11 and HisF8 provide bis-histidyl heme hexa-coordination in the globin domain of Geobacter sulfurreducens globin-coupled sensor
Articolo
Data di Pubblicazione:
2009
Citazione:
HisE11 and HisF8 provide bis-histidyl heme hexa-coordination in the globin domain of Geobacter sulfurreducens globin-coupled sensor / A. Pesce, L. Thijs, M. Nardini, F. Desmet, L. Sisinni, L. Gourlay, A. Bolli, M. Coletta, S. Van Doorslaer, X. Wan, M. Alam, P. Ascenzi, L. Moens, M. Bolognesi, S. Dewilde. - In: JOURNAL OF MOLECULAR BIOLOGY. - ISSN 0022-2836. - 386:1(2009), pp. 246-260.
Abstract:
Among heme-based sensors, recent phylogenomic and sequence analyses have identified 34 globin coupled sensors (GCS), to which an aerotactic or gene-regulating function has been tentatively ascribed. Here, the structural and biochemical characterization of the globin domain of the GCS from Geobacter sulfurreducens (GsGCS162) is reported. A combination of X-ray crystallography (crystal structure at 1.5 Å resolution), UV-Vis and Resonance Raman spectroscopy reveals the ferric GsGCS162 as an example of bis-histidyl hexa-coordinated GCS. At difference from known hexa-coordinated globins, the distal heme-coordination in ferric GsGCS162 is provided by a His residue unexpectedly located at the E11 topological site. Furthermore, UV-Vis and Resonance Raman spectroscopies indicate that ferrous deoxygenated GsGCS162 is a penta-/hexa-coordinated mixture, and the heme hexa-to-penta-coordination transition does not represent a rate-limiting step for carbonylation kinetics. Lastly, electron paramagnetic resonance indicates that ferrous nitrosylated GsGCS162 is a penta-coordinated species, where the proximal HisF8-Fe bond is severed.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
globin coupled sensor ; 3D structure ; ligand binding properties ; hexacoordination ; Geobacter sulfurreducens
Elenco autori:
A. Pesce, L. Thijs, M. Nardini, F. Desmet, L. Sisinni, L. Gourlay, A. Bolli, M. Coletta, S. Van Doorslaer, X. Wan, M. Alam, P. Ascenzi, L. Moens, M. Bolognesi, S. Dewilde
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