Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State
Capitolo
Data di Pubblicazione:
2021
Citazione:
Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State / P. Cocomazzi, L. Sorrentino, F. Cossu, A. Aliverti (METHODS IN MOLECULAR BIOLOGY). - In: Flavins and Flavoproteins / [a cura di] M. Barile. - Prima edizione. - New York : Springer, 2021. - ISBN 9781071612859. - pp. 179-187 [10.1007/978-1-0716-1286-6_11]
Abstract:
To perform their action, flavoproteins usually interact with a variety of low molecular weight partners, including electron transporters, yielding transient complexes whose tightness is often controlled by the redox state of the bound flavin cofactor. As a case study, here we describe the quantitative analysis of the redox-dependent interaction of the mammalian apoptosis inducing factor (AIF) with its NAD+ ligand. In particular, we report a protocol for the spectrophotometric titration of AIF in its reduced state under anaerobic conditions with NAD+, in order to determine the dissociation constant of the resulting complex.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Protein–ligand interaction; Dissociation constant; Electron carrier; Charge-transfer complex; Anaerobiosis; Photoreduction; Spectrophotometric titration
Elenco autori:
P. Cocomazzi, L. Sorrentino, F. Cossu, A. Aliverti
Link alla scheda completa:
Titolo del libro:
Flavins and Flavoproteins