Assess the utility of the PIP-ON system in M. tb and overexpress or silence several mycobacterial genes
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Data di Pubblicazione:
2007
Citazione:
Assess the utility of the PIP-ON system in M. tb and overexpress or silence several mycobacterial genes / F. Forti, D. Ghisotti. - [s.l] : null, 2007.
Abstract:
In this system the gene of interest is under transcriptional control of the Streptomyces Pip protein, the pristinamycin sensitive repressor. The pip gene of S. coelicolor has been cloned downstream of a constitutive mycobacterial promoter (the mutant pfurA102 of M. tb), and introduced in Mycobacterium smegmatis (see pMYS696 in Fig. 1). Furthermore, a Pip-ON system has been constructed, by cloning the S. pristinaespiralis ptr promoter upstream of a reporter gene (lacZ), in the above plasmid (see pMY718 in Fig. 1). These constructs have been introduced in M. smegmatis and beta-galactosidase activity measured: the activity expressed from pMY718 is very low (less than 10 Miller units) and inducible, in a dose dependent manner, by addition of pristinamycin.
Tipologia IRIS:
08 - Relazione interna o rapporto di ricerca
Elenco autori:
F. Forti, D. Ghisotti
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