Drinking water quality: An in vitro approach for the assessment of cytotoxic and genotoxic load in water sampled along distribution system
Articolo
Data di Pubblicazione:
2009
Citazione:
Drinking water quality: An in vitro approach for the assessment of cytotoxic and genotoxic load in water sampled along distribution system / F. Maffei, F. Carbone, G.C. Forti, A. Buschini, P. Poli, C. Rossi, L. Marabini, S. Radice, E. Chiesara, P. Hrelia. - In: ENVIRONMENT INTERNATIONAL. - ISSN 0160-4120. - 35:7(2009), pp. 1053-1061. [10.1016/j.envint.2009.05.007]
Abstract:
An in vitro approach was performed to assess the quality of drinking water collected at two treatment/
distribution networks located near the source (Plant #1) and the mouth of River Po (Plant #2). The water
was sampled at different points of each distribution network, before (raw water) and after the chlorine
dioxide disinfection, and in two points of the pipeline system to evaluate the influence of the distribution
system on the amount and quality of the disinfection by-product. Cytotoxicity and genotoxicity of water
extracts were evaluated in human peripheral lymphocytes and Hep-G2 cells by the use of the micronucleus
(MN) test and Comet assay. Raw water samples of both plants induced cytotoxic effects, but not the increases
of MN frequency in Hep-G2 cells and in human lymphocytes. Increases of DNA damage in human leukocytes
was detected by Comet assay for raw water of Plant #2 at concentration ≥0.25 Leq/mL. The disinfection
process generally has reduced the toxicity of water samples, even if potential direct DNA-damaging
compounds have been detectable in drinking water samples. The proposal approach, if currently used
together with chemical analysis, can contribute to improve the monitoring drinking water.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Comet assay; Disinfection by-product; Drinking water; Health risk; Micronucleus test
Elenco autori:
F. Maffei, F. Carbone, G.C. Forti, A. Buschini, P. Poli, C. Rossi, L. Marabini, S. Radice, E. Chiesara, P. Hrelia
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