Data di Pubblicazione:
2021
Citazione:
An Algorithm for the Analysis of the 3D Spatial Organization of the Genome / F. Gregoretti, A. Cortesi, G. Oliva, B. Bodega, L. Antonelli (METHODS IN MOLECULAR BIOLOGY). - In: Capturing Chromosome Conformation / [a cura di] B. Bodega, C. Lanzuolo. - [s.l] : Springer, 2021. - ISBN 9781071606636. - pp. 299-320 [10.1007/978-1-0716-0664-3_17]
Abstract:
We present an algorithm, and its MATLAB implementation, based on mathematical methods to detect and localize 3D multicolor DNA FISH spots in fluorescence cell image z-stacks. This algorithm provides a method to measure the relative positioning of spots in the nucleus and inter-spot distances with the aim to enrich our understanding of the 3D spatial organization of the genome within the cell nucleus.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Fluorescence microscopy; Multicolor 3D-FISH; Nuclear organization; Image processing and analysis; Cellular and subcellular segmentation; Variational segmentation model; Mathematical morphology method
Elenco autori:
F. Gregoretti, A. Cortesi, G. Oliva, B. Bodega, L. Antonelli
Link alla scheda completa:
Titolo del libro:
Capturing Chromosome Conformation