Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth
Articolo
Data di Pubblicazione:
2021
Citazione:
Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth / M. Scali, A. Moscatelli, L. Bini, E. Onelli, R. Vignani, W. Wang. - In: PROTEIN JOURNAL. - ISSN 1572-3887. - 40:28 (Special Issue)(2021 Apr), pp. 205-222. [10.1007/s10930-021-09972-x]
Abstract:
Pollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efcient vesicular transport
system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis,
membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafcking
activities required for tip growth. Proteomic analysis was applied to identify Nicotiana tabacum Diferentially Abundant
Proteins (DAPs) after in vitro pollen tube treatment with membrane trafcking inhibitors Brefeldin A, Ikarugamycin and
Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identifed by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identifed
proteins were classifed according to biological processes, and most proteins were related to pollen tube energy metabolism,
including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modifcation and
actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to
energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Nicotiana tabacum (L.); Pollen tube; Proteome; Membrane; Trafcking Inhibitors; Diferentially Abundant Proteins (DAPs)
Elenco autori:
M. Scali, A. Moscatelli, L. Bini, E. Onelli, R. Vignani, W. Wang
Link alla scheda completa: