A comparative morphological study of the response to ionizing rays in organotypic cultures of human skin and oral mucosa
Articolo
Data di Pubblicazione:
2009
Citazione:
A comparative morphological study of the response to ionizing rays in organotypic cultures of human skin and oral mucosa / E. Donetti, M. Bedoni, A. Gualerzi, P. Capone, G. Tartaglia, C. Sforza. - In: EXPERIMENTAL DERMATOLOGY. - ISSN 0906-6705. - 18:(2009), pp. 1086-1086. (Intervento presentato al 36. convegno Annual Meeting of Society for Cutaneous Ultrastructure Research tenutosi a Firenze nel 2009).
Abstract:
Mucositis is a frequent problem after irradiation of the oral mucosa. To study the early effects of irradiation on the desmosomal adhesion complex, explants of keratinized oral mucosa were exposed to a single dose of 2 Gy gamma irradiation. Biopsies were obtained from the upper dental arch of nine young healthy non-smoking women undergoing minor oral surgery. The biopsies were incubated in a Transwell culture system and, after irradiation, fixed in formalin 24 h later. Morphometric measurements of epithelial thickness revealed that it was unaffected by exposure to ionizing rays. Immunofluorescence analysis of desmosomal cadherin expression (desmoglein 1/desmoglein 3) demonstrated that the distribution of desmoglein 1 was not affected, whereas the expression of desmoglein 3 decreased in the suprabasal layers of irradiated samples. It is suggested that this has consequences for the mechanical integrity of the mucosa and promotes the development of radiation mucositis.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Donetti, M. Bedoni, A. Gualerzi, P. Capone, G. Tartaglia, C. Sforza
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