Data di Pubblicazione:
2009
Citazione:
Annular pupil filter under shot-noise condition for linear and non linear microscopy / E. Ronzitti, G. Vicidomini, V. Caorsi, A. Diaspro. - In: OPTICS EXPRESS. - ISSN 1094-4087. - 17:8(2009 Apr 13), pp. 6867-6880. [10.1364/OE.17.006867]
Abstract:
The imaging performances of multiphoton excitation and confocal laser scanning microscopy are herby considered: in typical experimental imaging conditions, a small finite amount of photon reaches the detector giving shot-noise fluctuations which affects the signal acquired. A significant detriment in the high frequencies transmission capability is obtained. In order to partially recover the high frequencies information lost, the insertion of a pupil plane filter in the microscope illumination light pathway on the objective lens is proposed. We demonstrate high-frequency and resolution enhancement in the case of linear and non linear fluorescence microscope approach under shot-noise condition.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Ronzitti, G. Vicidomini, V. Caorsi, A. Diaspro
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