Characterization of a repetitive element polymorphism - polymerase chain reaction chromosomal marker that discriminates Bacillus anthracis from related species
Articolo
Data di Pubblicazione:
2002
Citazione:
Characterization of a repetitive element polymorphism - polymerase
chain reaction chromosomal marker that
discriminates Bacillus anthracis from related species / A. Cherif, S. Borin, A. Rizzi, H. Ouzari, A. Boudabous, D. Daffonchio. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - 93:3(2002), pp. 456-462. [10.1046/j.1365-2672.2002.01712.x]
Abstract:
Aims: To identify a chromosomal marker with signature nucleotides specific for Bacillus
anthracis.
Methods and Results: Repetitive element polymorphism-polymerase chain reaction with
BOX-A1R primer was used to discriminate 52 strains of all six species of the B. cereus group .
A B. anthracis signature fragment, named AC-390, was cloned and sequenced. The deduced
amino acid sequence was homologous to that of YwfK of B. subtilis. Using two internal primers,
the AC-390 fragment was sequenced from two other B. anthracis strains as well as from strains
of B. cereus and B. thuringiensis which have an AC-390 fragment homologous to that of B.
anthracis as shown by Southern hybridization experiments.
Conclusions: Two new signature sequences specific for B. anthracis were identified on a
chromosomal fragment homologous to YwfK, a transcriptional regulator of B. subtilis.
Significance and Impact of the Study: These results show a new chromosomal DNA trait
useful for distinguishing B. anthracis from the related species of the B. cereus group, regardless
of the presence of the virulence plasmids pXO1 and pXO2.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Cherif, S. Borin, A. Rizzi, H. Ouzari, A. Boudabous, D. Daffonchio
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