Bacillus anthracis Diverges from Related Clades of the Bacillus cereus Group in 16S-23S Ribosomal DNA Intergenic Transcribed Spacers Containing tRNA Genes
Articolo
Data di Pubblicazione:
2003
Citazione:
Bacillus anthracis Diverges from Related Clades of the Bacillus cereus
Group in 16S-23S Ribosomal DNA Intergenic Transcribed
Spacers Containing tRNA Genes / A. Cherif, S. Borin, A. Rizzi, H. Ouzari, A. Boudabous, D. Daffonchio. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - 69:1(2003), pp. 33-40. [10.1128/AEM.69.1.33-40.2003]
Abstract:
Mung bean nuclease treatment of 16S–23S ribosomal DNA intergenic transcribed spacers (ITS) amplified
from several strains of the six species of the Bacillus cereus group showed that B. anthracis Davis TE702 and
B. mycoides G2 have other intermediate fragments in addition to the 220- and 550-bp homoduplex fragments
typical of the B. cereus group. Long and intermediate homoduplex ITS fragments from strains Davis TE702 and
G2 and from another 19 strains of the six species were sequenced. Two main types of ITS were found, either
with two tRNA genes (tRNAIle and tRNAAla) or without any at all. Strain Davis TE702 harbors an additional
ITS with a single tRNA gene, a hybrid between the tRNAIle and tRNAAla genes, suggesting that a recombination
event rather than a deletion generated the single tDNA-containing ITS. Strain G2 showed an additional ITS
of intermediate length with no tDNA and no similarity to other known sequences. Neighbor-joining analysis of
tDNA-containing long ITS indicated that B. cereus and B. thuringiensis represent a single clade. Three signature
sequences discriminated B. anthracis from B. cereus and B. thuringiensis, indicating that the anthrax agent
started evolving separately from the related clades of the B. cereus group. B. mycoides and B. weienstephanensis
were very closely related, while B. pseudomycoides appeared the most distant species.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Cherif, S. Borin, A. Rizzi, H. Ouzari, A. Boudabous, D. Daffonchio
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