Genetic relationship in the "Bacillus cereus group" by rep-PCR fingerprinting and sequencing of a Bacillus anthracis-specific rep-PCR fragment
Articolo
Data di Pubblicazione:
2003
Citazione:
Genetic relationship in the "Bacillus cereus group"
by rep-PCR fingerprinting and sequencing
of a Bacillus anthracis-specific rep-PCR fragment / A. Cherif, L. Brusetti, S. Borin, A. Rizzi, A. Boudabous, H. Khyami-Horani, D. Daffonchio. - In: JOURNAL OF APPLIED MICROBIOLOGY. - ISSN 1364-5072. - 94:6(2003), pp. 1108-1119. [10.1046/j.1365-2672.2003.01945.x]
Abstract:
Aims: To evaluate the genetic relationship in the Bacillus cereus group by rep-PCR fingerprinting.
Methods and Results: A collection of 112 strains of the six species of the B. cereus group was analysed by rep-
PCR fingerprinting using the BOX-A1R primer. A relative genetic distinctness was found among the species.
Cluster analysis of the rep-PCR patterns showed clusters of B. thuringiensis strains quite separate from those of
B. cereus strains. The B. anthracis strains represented an independent lineage in a B. cereus cluster. The B. mycoides,
B. pseudomycoides and B. weihenstephanensis strains were clustered into three groups at some distance from the other
species. Comparison of sequences of AC-390, a typical B. anthracis rep-PCR fragment, from 27 strains of
B. anthracis, B. cereus, B. thuringiensis and B. weihenstephanensis, representative of different clusters identified by
rep-PCR fingerprinting, confirmed that B. anthracis diverges from its related species.
Conclusions: The genetic relationship deduced from the rep-PCR patterns indicates a relatively clear separation of
the six species, suggesting that they can indeed be considered as separate units.
Significance and Impact of the Study: rep-PCR fingerprinting can make a contribution in the clarification of
the genetic relationships between the species of the B. cereus group.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Bacillus anthracis; Bacillus cereus group; BOX-A1R; Genetic relationship; rep-PCR; ywfK
Elenco autori:
A. Cherif, L. Brusetti, S. Borin, A. Rizzi, A. Boudabous, H. Khyami Horani, D. Daffonchio
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