Design of new chromogenic substrates for the spectrophotometric assay of lipolytic activity of lipases
Articolo
Data di Pubblicazione:
2006
Citazione:
Design of new chromogenic substrates for the spectrophotometric assay of lipolytic activity of lipases / E. Santaniello, P. Ciuffreda, S. Casati, H. El Sayed. - In: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC. - ISSN 1381-1177. - 40:3-4(2006), pp. 76-80.
Abstract:
The lipolytic activity of lipases from porcine pancreas (PPL) and Pseudomonas cepacia (PCL) on synthetic dialkylglycerol fatty acyl esters was evaluated by titrimetric method and the observed chain length selectivity constituted the basis for the design of new chromogenic substrates [1,2-di-O-butyl-sn-glycerol-3-(4-nitrophenyl)carbonate and 1,2-di-O-octyl-sn-glycerol-3-(4-nitrophenyl)glutarate] that were shown to be specific for spectrophotometric assay of PPL and PCL activity, respectively. (copyright) 2006 Elsevier B.V. All rights reserved.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
4-nitrophenyl esters; dialkylglycerol esters; lipolytic activity; lipases; spectrophotometric assay
Elenco autori:
E. Santaniello, P. Ciuffreda, S. Casati, H. El Sayed
Link alla scheda completa: