Human polymorphonuclear leukocytes produce and express functional tissue factor upon stimulation
Articolo
Data di Pubblicazione:
2006
Citazione:
Human polymorphonuclear leukocytes produce and express functional tissue factor upon stimulation / N. MAUGERI, M. BRAMBILLA, M. CAMERA, A. CARBONE, E. TREMOLI, M.B. DONATI, G. DE GAETANO, C. CERLETTI. - In: JOURNAL OF THROMBOSIS AND HAEMOSTASIS. - ISSN 1538-7933. - 4:6(2006), pp. 1323-1330. [10.1111/j.1538-7836.2006.01968.x]
Abstract:
Background: Blood-borne tissue factor (TF) plays a crucial role in thrombogenesis. Aim: To study whether polymorphonuclear leukocytes (PMN) are a source of TF. Methods and Results: Human PMN were carefully separated from ot her blood cells and stimulated for 3 min with purified P-selectin or the chemotactic peptide formyl-MetLeu- Phe (fMLP): they expressed both TF procoagulant activity, as identified by specific TF MoAb and inactivated factor VIIa blockade; and TF:Ag (four to six times), as shown by flowcytometry and immunocytochemistry. About 40% of permeabilized PMN, both resting and stimulated, contained TF:Ag, indicating that stimulation onlymodifies the location of TF:Ag within PMN. By real time-polymerase chain reaction (RT-PCR), a very low amount of TF mRNA was detectable in resting PMN, but a 3- to 5-fold increase was observed after 1-h stimulation with P-selectin or fMLP, respectively. Conclusions: These findings suggest that TF is not constitutively expressed in peripheral PMN, but can be up-regulated and produced upon stimulation and specific gene transcription, as for instance during contact with activated platelets or endothelium. The stored TF is rapidly expressed in vitro as a functional molecule on the surface of activated PMN. The availability of PMNTF supports the relevance of inflammatory cells and their interactionwith platelets for fibrin deposition and thrombus formation
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Inflammation; Platelet P-selectin; Polymorphonuclear leukocytes; Thrombosis; Tissue factor
Elenco autori:
N. Maugeri, M. Brambilla, M. Camera, A. Carbone, E. Tremoli, M.B. Donati, G. DE GAETANO, C. Cerletti
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