Deletion of PDE2, the gene encoding the high-affinity cAMP phosphodiesterase, results in changes of the cell wall and membrane in Candida albicans
Articolo
Data di Pubblicazione:
2005
Citazione:
Deletion of PDE2, the gene encoding the high-affinity cAMP phosphodiesterase, results in changes of the cell wall and membrane in Candida albicans / W.H. Jung, P. Warn, E. Ragni, L. Popolo, C.D. Nunn, M.P. Turner, L. Stateva. - In: YEAST. - ISSN 0749-503X. - 22:4(2005), pp. 285-294. [10.1002/yea.1199]
Abstract:
Abstract
A role for the cAMP-dependent pathway in regulation of the cell wall in the model
yeast Saccharomyces cerevisiae has recently been demonstrated. In this study we
report the results of a phenotypic analysis of a Candida albicans mutant, characterized
by a constitutive activation of the cAMP pathway due to deletion of PDE2, the gene
encoding the high cAMP-affinity phosphodiesterase. Unlike wild-type strains, this
mutant has an increased sensitivity to cell wall and membrane perturbing agents
such as SDS and CFW, and antifungals such as amphotericin B and flucytosine.
Moreover, the mutant is characterized by an altered sensitivity and a significantly
reduced tolerance to fluconazole. The mutant’s membrane has around 30% higher
ergosterol content and the cell wall glucan was 22% lower than in the wild-type.
These cell wall and membrane changes are manifested by a considerable reduction in
the thickness of the cell wall, which in the mutant is on average 60–65 nm, compared
to 80–85 nm in the wild-type strains as revealed by electron microscopy. These
results suggest that constitutive activation of the cAMP pathway affects cell wall
and membrane structure, and biosynthesis, not only in the model yeast S. cerevisiae
but also in the human fungal pathogen C. albicans. Copyright 2005 John Wiley &
Sons, Ltd.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Candida albicans; cAMP-dependent pathway; PDE2; cell wall; antifungal
susceptibility
Elenco autori:
W.H. Jung, P. Warn, E. Ragni, L. Popolo, C.D. Nunn, M.P. Turner, L. Stateva
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