Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus
Articolo
Data di Pubblicazione:
2001
Citazione:
Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus / A. Pesce, M. Nardini, S. Dewilde, P. Ascenzi, A.F. Riggs, K. Yamauchi, E. Geuens, L. Moens, M. Bolognesi. - In: ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. - ISSN 0907-4449. - 57:12(2001), pp. 1897-1899. [10.1107/S0907444901015815]
Abstract:
The nemertean worm Cerebratulus lacteus neural tissue haemoglobin (109 amino acids, the shortest known haemoglobin) has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant native data set has been collected at the Cu Kα wavelength to 2.05 Å resolution. The crystals belong to the orthorhombic P212121 space group, with unit-cell parameters a = 42.5, b = 43.1, c = 60.2 Å and one molecule per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe atom, thus paving the way for MAD/SAD structure determination.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
A. Pesce, M. Nardini, S. Dewilde, P. Ascenzi, A.F. Riggs, K. Yamauchi, E. Geuens, L. Moens, M. Bolognesi
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