Lead-resistant microorganisms from marble red stains of Certosa of Pavia, Italy and use of nucleic acid-based techniques for their detection
Articolo
Data di Pubblicazione:
1997
Citazione:
Lead-resistant microorganisms from marble red stains of Certosa of Pavia, Italy and use of nucleic acid-based techniques for their detection / E. Zanardini, V. Andreoni, S. Borin, F. Cappitelli, D. Daffonchio, P. Talotta, C. Sorlini, G. Ranalli, S. Bruni, F. Cariati. - In: INTERNATIONAL BIODETERIORATION & BIODEGRADATION. - ISSN 0964-8305. - 40:2-4(1997), pp. 171-182.
Abstract:
Twenty-one microbial strains have been isolated from the red spots present on the
marble of the facade of the Certosa of Pavia. These strains have been characterized
and identified to the genus level using API test kits. As the pigmentation of these
stains was previously attributed to minium (PbsOd), the resistance to lead of the
strains isolated was tested in a liquid medium. Thirteen strains showed resistance to
lead nitrate at concentrations up to 500 ppm, while four, Pseudomonas vesicularis,
Bacillus cereus, Staphylococcus sp. and Streptomyces sp., grew normally at 1300 ppm
and with difficulty at 1500ppm. In the presence of lead nitrate P. vesicularis and
Streptomyces sp. showed a red and red-brown pigmentation in the cultures,
respectively. The EDX analysis demonstrated that lead was associated with
Streptomyces cells. Further investigations were carried out to assay the suitability
of rapid molecular tests for the detection of isolates responsible for red stains directly
on the biodeteriorated samples. The polymerase chain reaction technique was used
on fifteen altered samples of artworks of different origins to detect B. cereus, chosen
as test organism to set up the analytical method. The technique allowed for the
detection of the organism in less than 24 h, including a 12-h enrichment step. Among
the samples analyzed, B. cereus was detected via PCR only in samples from which it
had been previously isolated with conventional techniques, i.e. samples from the
Certosa of Pavia and Ca’ D’Oro in Venice. The results indicate the potential
usefulness of the molecular approach for the detection of alterative microorganisms
on artworks. The advantages of the method in terms of speed, specificity and
sensitivity in comparison with conventional ones are discussed.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
E. Zanardini, V. Andreoni, S. Borin, F. Cappitelli, D. Daffonchio, P. Talotta, C. Sorlini, G. Ranalli, S. Bruni, F. Cariati
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