Data di Pubblicazione:
2018
Citazione:
Preparation of anti-miR PNAs for drug development and nanomedicine / A. Manicardi, R. Gambari, L. de Cola, R. Corradini (METHODS IN MOLECULAR BIOLOGY). - In: DNA Nanotechnology : Methods and Protocols / [a cura di] G. Zuccheri. - [s.l] : Humana Press Inc., 2018. - ISBN 9781493985821. - pp. 49-63 [10.1007/978-1-4939-8582-1_4]
Abstract:
Peptide Nucleic Acids (PNAs) are oligonucleotide mimics that can be used to block the biological action of microRNA, thus affecting gene expression post-transcriptionally. PNAs are obtained with solid-phase peptide synthesis, and can be easily conjugated to other peptides. Conjugation with R8-Peptide or modification of the PNA backbone (at C5 or C2 carbon) with arginine side chains allows efficient cellular uptake. The present protocol describes the synthesis of cationic PNAs that can be used alone as drugs or for efficient co-delivery in suitable inorganic nanocarriers.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
PNA; Backbone-modified PNA; Peptide carrier; Solid-phase synthesis; Drug delivery
Elenco autori:
A. Manicardi, R. Gambari, L. de Cola, R. Corradini
Link alla scheda completa:
Titolo del libro:
DNA Nanotechnology : Methods and Protocols