A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize
Articolo
Data di Pubblicazione:
2002
Citazione:
A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize / M.-X. Sun, E. Kranz, A. Moscatelli, H.-Y. Yang, H. Lorz, M. Cresti. - In: SEXUAL PLANT REPRODUCTION. - ISSN 0934-0882. - 15:1(2002), pp. 53-55.
Abstract:
A novel protocol for direct detection and localization of lectin binding sites on the surface of single sperm cells is presented. Fluorescence-conjugated lectins and single cell manipulation techniques were employed in this protocol. Advantages of the protocol include that sperm cell membranes are well protected and artifacts avoided by minimizing movement of the medium. Damage of the sperm membrane was reduced using a droplet-staining/washing method. Any morphological change may be readily recorded.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
In vitro fertilization; Keywords Sperm cells; Lectin binding sites; Maize
Elenco autori:
M.-X. Sun, E. Kranz, A. Moscatelli, H.-Y. Yang, H. Lorz, M. Cresti
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