Expression of MMP-9 and TIMP-2 in feline diffuse iris melanoma : correlation with histological grade and mitotic index
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Data di Pubblicazione:
2018
Citazione:
Expression of MMP-9 and TIMP-2 in feline diffuse iris melanoma : correlation with histological grade and mitotic index / L. Nordio, V. Stornelli, C. Giudice. ((Intervento presentato al convegno ESVP-ECVP Annual Meeting tenutosi a Cluj-Napoca, Romania nel 2018.
Abstract:
Introduction: Feline diffuse iris melanoma (FDIM) is the most common
feline primary intraocular neoplasm. It is a slowly progressive malignant
tumour, representing a possiblemodel of human iris melanoma.
In cats, histological grade andmitotic index (MI) are considered predictors
of survival. Matrix metalloproteinase-9 (MMP-9) is an endopeptidase
able to digest the extracellular matrix and is involved in tumour
invasion. Its specific inhibitor is the tissue inhibitor of metalloproteinases-
2 (TIMP-2). The present study investigates the correlation between
MMP-9/TIMP-2 expression and histological grade and MI.
Materials and Methods: Sixty-one FDIMs were graded on light microscopy
(grade I, n 5 22; grade II, n 5 20; grade III, n 5 19) and MI
was calculated. Immunohistochemistry was performed using monoclonal
anti-MMP-9 and anti-TIMP-2 antibodies and scored semiquantitatively.
Results were compared by the Mann-Whitney U test.
Results: MMP-9 was expressed in 59.1% grade I FDIMs, 90.0% grade
II and 80.0% grade III tumours.TIMP-2 immunolabelled 59.1% grade
I, 80.0% grade II and 73.7% grade IIIFDIMs.MMP-9was expressed in
71.4% of FDIMswithMI7 and 92.3% of FDIMwithMIO7. TIMP-
2 was detected in 67.3% of caseswithMI7 and 76.9% of caseswithMI
O7. MMP-9 and TIMP-2 expression differed significantly between
grade I and the other two grades. TIMP-2 expression was correlated
withMMP-9 expression in grade I and III tumours. IntenseMMP-9,
but not TIMP-2, was significantly associated with high MI.
Conclusions: In FDIM intense immunohistochemical expression of
MMP-9 correlated with known histological parameters of aggressiveness,
specifically histological grade and MI.
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
L. Nordio, V. Stornelli, C. Giudice
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