Data di Pubblicazione:
2020
Citazione:
From Protein Toxins to Applied Toxicological Testing / L. Palazzolo, E. Gianazza, I. Eberini. ((Intervento presentato al convegno PATB Committee Protein Toxins Workshop: "From Protein Toxins to Applied Toxicological Testing" tenutosi a Virtual nel 2020.
Abstract:
Topics for Discussion (~ 40 min. per topic)
1. Is a harmonized toxin database needed? (i.e, a single DB)
a) Toxins vs. allergens; DB usefulness vs. toxicity information
b) Toxicity is defined by protein function; allergenicity is defined by IgE binding (not
depending on protein function)
c) Input from Toxin biologists
d) Do we need an additional database or Do we need a Tool to navigate across all
databases already available?
2. Toxin Definition
a) How to decide what goes into a DB (criteria)? (e.g., nature – mammalian toxins?)
b) Toxin/anti-toxin (how does anti-toxins fit the definition? Why would they be in a DB?)
c) Sorting of toxins (level of toxicity) - target organism would impact sorting; impact of
“effectors”.
3. Toxin Sequence List/Database
a) Continuation of Day-2 discussion / Comparison with Industry DBs
4. Tools for assessing toxins
a) Sequence similarities
b) Thresholds
i. Does this apply to engineered proteins? What if 3 aa changes?
c) Tools (single or series of tools)
d) Best ways to use primary sequence & structural data to predict toxins? (e.g., Pfam)
e) Where bioinformatics could take us? (could inform functionality; could also inform
about alignments). Understanding limitations of tools
5. Back to first question: Is a harmonized toxin database needed?
6. Future steps - where do we go from here?
a) 3Rs? (Refine, Replace, Reduce)
b) Tier-1 (ID hazard) & Tier-2 (characterize hazard; tox studies) - (Delaney paper)
i. Is tier-2 always needed? What factors prompt a “go/no go decision” for tier-2? (future steps)?
Tipologia IRIS:
14 - Intervento a convegno non pubblicato
Elenco autori:
L. Palazzolo, E. Gianazza, I. Eberini
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