Data di Pubblicazione:
2012
Citazione:
Direct imaging of DNA fibers : the visage of double helix / F. Gentile, M. Moretti, T. Limongi, A. Falqui, G. Bertoni, A. Scarpellini, S. Santoriello, L. Maragliano, R. Proietti Zaccaria, E. Di Fabrizio. - In: NANO LETTERS. - ISSN 1530-6984. - 12:12(2012 Nov), pp. 6453-6458. [10.1021/nl3039162]
Abstract:
Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
DNA bundles direct imaging; molecular dynamics simulations; nanofabricated micropillars; superhydrophobic surface; transmission electron microscopy; Bacteriophage lambda; DNA, Viral; Hydrophobic and Hydrophilic Interactions; Molecular Dynamics Simulation; Nanofibers; Nucleic Acid Conformation
Elenco autori:
F. Gentile, M. Moretti, T. Limongi, A. Falqui, G. Bertoni, A. Scarpellini, S. Santoriello, L. Maragliano, R. Proietti Zaccaria, E. Di Fabrizio
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