Data di Pubblicazione:
2020
Citazione:
Covalent grafting of antimicrobial peptides onto microcrystalline cellulose / P. Sperandeo, F. Bosco, F. Clerici, A. Polissi, M.L. Gelmi, A. Romanelli. - In: ACS APPLIED BIO MATERIALS. - ISSN 2576-6422. - 3:8(2020 Aug 17), pp. 4895-4901.
Abstract:
The purpose of this work is to set up a generalprotocol for the production of antimicrobial materials based oncellulose and peptides. We exploited the chemical ligation reactionto achieve the conjugation of peptides to cellulose; to this aim, weproduced thioester peptides and cysteine-modified cellulose. Asthe thioester handle can be inserted at any position of the peptide,the peptide can be immobilized onto the cellulose through its N-or C-terminal end or through any other position within thesequence. Our experiments performed onEscherichia coliculturesshow that the cellulose conjugated to the peptides lasioglossin-IIIand TBKKG6A causes a significant reduction in the concentrationof viable cells as compared to unmodified cellulose. In conclusion, antimicrobial peptides bound to cellulose through a covalent bondretain their activity and therefore have the potential to be used as active ingredients in antimicrobial materials.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
peptide, cellulose, conjugation, chemical ligation, antimicrobial, E. coli
Elenco autori:
P. Sperandeo, F. Bosco, F. Clerici, A. Polissi, M.L. Gelmi, A. Romanelli
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