Autoantibody Profile of a Cohort of 54 Italian Patients with Linear IgA Bullous Dermatosis: LAD-1 Denoted as a Major Auto-antigen of the Lamina Lucida Subtype
Articolo
Data di Pubblicazione:
2020
Citazione:
Autoantibody Profile of a Cohort of 54 Italian Patients with Linear IgA Bullous Dermatosis: LAD-1 Denoted as a Major Auto-antigen of the Lamina Lucida Subtype / E. Cozzani, G. Di Zenzo, G. Gasparini, A. Salemme, A.F. Agnoletti, C. Vassallo, M. Caproni, E. Antiga, A.V. Marzano, R. Cavalli, C. Ocella, C. de Simone, A. Parodi. - In: ACTA DERMATO-VENEREOLOGICA. - ISSN 0001-5555. - 100:4(2020 Feb), pp. adv00070.1-adv00070.5. [10.2340/00015555-3415]
Abstract:
Linear IgA bullous dermatosis (LABD) is characterized by presence of multiple IgA autoantibodies, and a comparatively lesser number of IgG antibodies, directed against different hemidesmosomal antigens. The main autoantigens are LAD-1, LABD-97, BP180 and BP230, type VII collagen and laminin 332. We retrospectively studied the serology of 54 Italian patients with LABD using enzyme-linked immunosorbent assay (ELISA), immunoblotting assay, and indirect immunofluorescence on monkey oesophagus and salt-split skin. Among these, indirect immunofluorescence of salt-split skin elicits the greatest sensitivity. Sixty-three percent of the sera were observed to be positive, with a lamina lucida pattern observed in 48%, a sub-lamina densa pattern in 2% and a mixed pattern in 13% of the cases. IgA reactivity to LAD-1 on immunoblotting was found in 52% of sera, to BP180-NC16A by ELISA in 32% and to BP230 in 26%. Only 17% of patients possessed circulating IgG autoantibodies. LAD-1 was determined to be a major autoantigen of the lamina lucida subtype. Combined serological assays demonstrated a high sensitivity (82%), suggesting that this approach could support diagnosis when a biopsy is not feasible or direct immunofluorescence results are negative.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
BP180; BP230; diagnostic sensitivity; humoral immune response; LAD-1; linear IgA bullous disease
Elenco autori:
E. Cozzani, G. Di Zenzo, G. Gasparini, A. Salemme, A.F. Agnoletti, C. Vassallo, M. Caproni, E. Antiga, A.V. Marzano, R. Cavalli, C. Ocella, C. de Simone, A. Parodi
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