Transcriptional Regulation of Sorghum Stem Composition : Key Players Identified Through Co-expression Gene Network and Comparative Genomics Analyses
Articolo
Data di Pubblicazione:
2020
Citazione:
Transcriptional Regulation of Sorghum Stem Composition : Key Players Identified Through Co-expression Gene Network and Comparative Genomics Analyses / L. Hennet, A. Berger, N. Trabanco, E. Ricciuti, J. Dufayard, S. Bocs, D. Bastianelli, L. Bonnal, S. Roques, L. Rossini, D. Luquet, N. Terrier, D. Pot. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 11(2020 Mar 03), pp. 224.1-224.29. [10.3389/fpls.2020.00224]
Abstract:
Most sorghum biomass accumulates in stem secondary cell walls (SCW). As sorghum
stems are used as raw materials for various purposes such as feed, energy and
fiber reinforced polymers, identifying the genes responsible for SCW establishment
is highly important. Taking advantage of studies performed in model species, most
of the structural genes contributing at the molecular level to the SCW biosynthesis
in sorghum have been proposed while their regulatory factors have mostly not been
determined. Validation of the role of several MYB and NAC transcription factors in
SCW regulation in Arabidopsis and a few other species has been provided. In this
study, we contributed to the recent efforts made in grasses to uncover the mechanisms
underlying SCW establishment. We reported updated phylogenies of NAC and MYB
in 9 different species and exploited findings from other species to highlight candidate
regulators of SCW in sorghum. We acquired expression data during sorghum internode
development and used co-expression analyses to determine groups of co-expressed
genes that are likely to be involved in SCW establishment. We were able to identify two
groups of co-expressed genes presenting multiple evidences of involvement in SCW
building. Gene enrichment analysis of MYB and NAC genes provided evidence that
while NAC SECONDARY WALL THICKENING PROMOTING FACTOR NST genes and
SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN gene functions appear to
be conserved in sorghum, NAC master regulators of SCW in sorghum may not be as
tissue compartmentalized as in Arabidopsis. We showed that for every homolog of the
key SCW MYB in Arabidopsis, a similar role is expected for sorghum. In addition, we
unveiled sorghum MYB and NAC that have not been identified to date as being involved
in cell wall regulation. Although specific validation of the MYB and NAC genes uncovered
in this study is needed, we provide a network of sorghum genes involved in SCW both
at the structural and regulatory levels.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
cell wall; gene co-expression network analysis; internode; phylogeny; transcription factor; NAC; MYB; sorghum
Elenco autori:
L. Hennet, A. Berger, N. Trabanco, E. Ricciuti, J. Dufayard, S. Bocs, D. Bastianelli, L. Bonnal, S. Roques, L. Rossini, D. Luquet, N. Terrier, D. Pot
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