Analysis of protein ions in the range 3000-12000 Th under partial (no discharge) atmospheric pressure chemical ionization conditions using ion trap mass spectrometry
Articolo
Data di Pubblicazione:
2002
Citazione:
Analysis of protein ions in the range 3000-12000 Th under partial (no discharge)
atmospheric pressure chemical ionization conditions using ion trap mass
spectrometry / S. Cristoni, L. Rossi Bernardi, I. Biunno, F. Guidugli. - In: RAPID COMMUNICATIONS IN MASS SPECTROMETRY. - ISSN 0951-4198. - 16:12(2002), pp. 1153-1159.
Abstract:
A new approach, based on the use of atmospheric pressure chemical ionization ion
trap mass spectrometry (APCI-ITMS), but without a corona discharge, was
investigated for application to creating and monitoring protein ions. It must be
emphasized that APCI is not usually used in protein analysis. In order to verify
the applicability of the proposed method to the analysis of proteins, two
standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A
mixture of the two proteins was also analyzed showing that this novel approach,
based on the use of APCI, can be used in the analysis of protein mixtures.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Cytochrome c Group
Ethylamines
Myoglobin
Solvents
triethylamine
Elenco autori:
S. Cristoni, L. Rossi Bernardi, I. Biunno, F. Guidugli
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