In vitro double fertilization in Nicotiana tabacum (L.): the role of cell volume in cell fusion
Articolo
Data di Pubblicazione:
2000
Citazione:
In vitro double fertilization in Nicotiana tabacum (L.): the role of cell volume in cell fusion / M.-X. Sun, A. Moscatelli, H.-Y. Yang, M. Cresti. - In: SEXUAL PLANT REPRODUCTION. - ISSN 0934-0882. - 13:4(2000), pp. 225-229.
Abstract:
The role of cell volume in regulating cell fusion was investigated by in vitro fertilization to elucidate mechanisms involved in double fertilization. The results revealed that in our model gamete fusion was more efficient than the fusion of somatic cells under the same conditions. The fusion of selected mesophyll protoplasts of different sizes and their fusion with chloroplasts demonstrated that cell volume ratio played a role in this process: as the ratio increased, fusion was more efficient and faster. When one of the cells was as small as a sperm, the formation of a round fusion product was faster. This might explain why gamete fusion was highly efficient in all in vitro germ cell fusion systems. This finding may also explain why sperm evolved as small cells. The results reported here will be useful for interpreting and evaluating data of in vitro fertilization experiments and for distinguishing gamete-specific characters.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
Nicotiana tabacum ; in vitro fertilization ; cell fusion ; cell volume ; membrane reorganization
Elenco autori:
M.-X. Sun, A. Moscatelli, H.-Y. Yang, M. Cresti
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