Oxygenase systems in an oligotrophic bacterial community of a subsurface water polluted by btex
Capitolo
Data di Pubblicazione:
2002
Citazione:
Oxygenase systems in an oligotrophic bacterial community of a subsurface water polluted by btex / L. Cavalca, E. Dell'Amico, V. Andreoni (DEVELOPMENTS IN SOIL SCIENCE). - In: Soil Mineral-Organic Matter-Microorganism Interactions and Ecosystem Health / [a cura di] L. Gianfreda, J.-M. Bollag, P. Ming Huang, A. Violante. - [s.l] : Elsevier, 2002. - ISBN 0444510397. - pp. 363-375 (( Intervento presentato al 3. convegno Symposium on Soil Mineral-Organic Matter-Microorganism Interactions and Ecosystem Health tenutosi a Napoli nel 2000.
Abstract:
Molecular probes were designed to detect genes coding for various oxygenase systems. PCR amplifications were carried out either on DNA extracted directly from water samples or from isolates. In the DNA from water samples, genes for toluene-benzene monoxygenase (tbmA), for toluene dioxygenase (todCl), for catechol 2,3-dioxygenase (xylE), and for catechol 1,2-dioxygenase (catA) were present. Among 100 strains isolated to check the presence of hydrocarbon-degrading bacteria, 11 grew on aromatic substrates and most of them attacked aromatics through monoxygenase enzymatic systems. In strains able to grow on benzene, the nucleotide sequence of the 580 bp PCR fragment showed 78% homology to the bmoB gene for benzene monoxygenase of Pseudomonas aeruginosa JI104. In strains able to grow on m-xylene and p-xylene, the PCR fragment showed 66% homology to the pheB gene for phenol hydroxylase of Ralstonia eutropha JMP134. Degradation patterns of isolates monitored by HPLC analysis were in accord with the presence of these degradative enzymes. The probes developed in the present study enlarge the detection of degradative systems in the environment.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
gene; toluene; Xylene; monooxygenase; degradation; catabolism; benzene; plasmid; ring; soil
Elenco autori:
L. Cavalca, E. Dell'Amico, V. Andreoni
Link alla scheda completa:
Titolo del libro:
Soil Mineral-Organic Matter-Microorganism Interactions and Ecosystem Health