Data di Pubblicazione:
2017
Citazione:
Clickable polymeric coating for glycan microarrays / C. Zilio, L. Sola, M. Cretich, A. Bernardi, M. Chiari (METHODS IN MOLECULAR BIOLOGY). - In: Small Molecule Microarrays / [a cura di] M. Uttamchandani, S.Q. Yao. - [s.l] : Humana Press, 2017. - ISBN 978-1-4939-6584-7. - pp. 55-66 [10.1007/978-1-4939-6584-7_5]
Abstract:
The interaction of carbohydrates with a variety of biological targets,including antibodies,proteins,viruses,and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. In this study,a novel microarray support is reported for the fabrication of glycan arrays that combines the higher sensitivity of a layered Si-SiO2surface with a novel polymeric coating easily modifiable by subsequent click reaction. The alkyne-containing copolymer,adsorbed from an aqueous solution,produces a coating by a single step procedure and serves as a soft,tridimensional support for the oriented immobilization of carbohydrates via azide/alkyne Cu (I) catalyzed "click" reaction. The advantages of a functional 3D polymer coating making use of a click chemistry immobilization are combined with the high fluorescence sensitivity and superior signal-to-noise ratio of a Si-SiO2substrate. The proposed approach enables the attachment of complex sugars on a silicon oxide surface by a method that does not require skilled personnel and chemistry laboratories.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
Click chemistry; Glycan microarray; Oriented immobilization; Polymeric coating
Elenco autori:
C. Zilio, L. Sola, M. Cretich, A. Bernardi, M. Chiari
Link alla scheda completa:
Titolo del libro:
Small Molecule Microarrays