Luteal estrogen receptor, interleukin-1, and apoptosis-associated genes during prostaglandin F2alpha-induced luteolysis in rabbits at different stages of pseudopregnancy
Abstract
Data di Pubblicazione:
2008
Citazione:
Luteal estrogen receptor, interleukin-1, and apoptosis-associated genes during prostaglandin F2alpha-induced luteolysis in rabbits at different stages of pseudopregnancy / M. Maranesi, L. Lilli, G. Brecchia, C. Dallaglio, G. Guelfi, M. Zerani, A. Gobbetti, B. C.. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 43:Suppl. 3(2008 Jul), pp. 154-154. ((Intervento presentato al 16. convegno International Congress on Animal Reproduction tenutosi a Budapest nel 2008.
Abstract:
In rabbits, several intraluteal pathways have emerged as potential mediators of the luteolytic action of prostaglandin F2αPGF2α, but the mechanisms that protect the developing corpora lutea (CL) from functional luteolysis to occur up to day 5 of pseudopregnancy are still unclear. Thus, the aim of this study was to elucidate PGF2α-induced temporal expression changes for estrogen receptor (ERα), interleukin-1(IL-1β), p53, and Bcl-x genes in day 4 vs. day 9 CL, in order to further characterize their role in the acquisition of luteal capacity to PGF2α. CL were collected from rabbits 0, 1.5, 3, 6, 12, and 24 h after 200 μg i.m. PGF2α (alfaprostol) injection given at either day 4 or 9 of pseudopregnancy induced by 0.8 μg i.m. GnRH administration. mRNA was extracted at different time points and multiplex RT-PCR technique was used to obtain semi-quantitative abundances of target genes. Before treatment, ERαmRNA steady state levels were lower (P<0.01) in day 4 than in day 9 CL. At day 4, luteal ERαmRNA levels remained fairly constant after treatment, whereas at day 9 gradually declined to low values (P<0.01) within 6 h after PGF2αinjection. At day 4, IL-1β mRNA transcript peaked (P≤0.01) two-fold 3 h after PGF2α challenge and thereafter gradually fell to pre-treatment values, whereas at day 9 the peak level was reached 6 h after PGF2α. Before treatment, Bcl-x transcripts were higher (P<0.01) in day 4 than in day 9 CL. At day 4, the Bcl-x mRNA levels gradually dropped within the first 3 h after PGF2α and then remained unchanged thereafter. At day 9, Bcl-x mRNA relative abundance decreased (P<0.01) 6 h after PGF2α administration to reach the lowest values (P<0.01) 24 h later. Pretreatment p53 mRNA values did not differ between luteal stages. At day 4, p53 mRNA progressively declined within the following 24 h after treatment. At day 9, p53 mRNA increased up to 3 h after PGF2α and then diminished. In conclusion, these findings suggest that PGF2α regulates luteolysis by ERαmRNA downregulation and by modulation of pro- and anti-apoptotic pathways in CL that have acquired luteolytic capacity.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M. Maranesi, L. Lilli, G. Brecchia, C. Dallaglio, G. Guelfi, M. Zerani, A. Gobbetti, B. C.
Link alla scheda completa: