Data di Pubblicazione:
2004
Citazione:
Experiences in the measurement of RBC-bound IgG as markers of cell age / R. Paleari, F. Ceriotti, F. Azzario, L. Maccioni, R. Galanello, A. Mosca. - In: BIOELECTROCHEMISTRY. - ISSN 1567-5394. - 62:2(2004), pp. 175-179. [10.1016/j.bioelechem.2003.07.006]
Abstract:
An immunologically mediated pathway has been largely accepted to be one of the mechanisms involved in the clearance of senescent or
prematurely damaged RBC. According to this pathway, RBC removal is mediated by binding of naturally occurring IgG to clustered integral
membrane proteins, followed by complement deposition. The validation of an immunoenzymatic method for the detection of RBC-bound
autologous IgG is presented. The use of RBC-bound IgG as an index related to red cell age was evaluated by measuring IgG binding in RBC
treated with the clustering agent ZnCl2, in density fractionated RBC and in a selected group of patients expected to have an altered RBC life
span. The immunoenzymatic method for IgG detection resulted to be reproducible (CV = 3.4%). IgG binding to in vitro clustered RBC was
found to be enhanced to a very great extent, about 20 times higher with respect to untreated RBC. A slight but significant increase (about 1.8-
fold) in membrane-bound IgG was observed in the highest density fraction of normal RBC, which constituted 1% of the total cells. A
significantly greater number of RBC-bound IgG was measured in splenectomized h-thalassemia intermedia patients and in subjects with
secondary decreases in the C3 complement fraction concentration.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
R. Paleari, F. Ceriotti, F. Azzario, L. Maccioni, R. Galanello, A. Mosca
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