Data di Pubblicazione:
2006
Citazione:
Guard cells : transcription factors regulate stomatal movements and water loss / E. Cominelli, M. Galbiati, T. Sala, C. Tonelli - In: Proceedings of the 50th Italian Society of Agricultural Genetics Annual Congress[s.l] : Società Italiana di Genetica Agraria, 2006. - ISBN 88-900622-7-4. - pp. 5.04-5.04 (( Intervento presentato al 50. convegno Convegno Annuale della Società Italiana di Genetica Agraria tenutosi a Ischia nel 2006.
Abstract:
The regulation of the opening of stomata allows the plant to cope with the conflicting needs of
ensuring a sufficient uptake of CO2 for photosynthesis and of minimizing water loss. Guard cells
integrate internal signals and environmental stimuli to modulate stomatal aperture for plant survival
under diverse conditions. Evidence suggests that modulation of transcription plays an important role
in controlling guard cell activity, even though the details of this level of regulation remain mostly
unknown. Recent work from different groups shows that two members of the R2-R3MYB
transcription factors family of Arabidopis thaliana, AtMYB60 and AtMYB61, are necessary for
stomatal movements. Here we report the characterization of AtMYB60 gene and of its promoter
sequence. AtMYB60 is specifically expressed in guard cell and its expression is negatively
modulated during drought. A null mutation in AtMYB60 results in the constitutive reduction of
stomatal opening and in decreased wilting under water stress conditions. Transcript levels of a
limited number of genes are altered in the mutant, many of which involved in the plant response to
stress. We also report the deletion analysis of AtMYB60 gene promoter through transgenic lines
harbouring different portions fused to the GUS reporter gene. We identified the minimal promoter
fragment necessary and sufficient for AtMYB60 gene expression in guard cells and some putative
regulators proteins binding to this portion. Our data indicate that AtMYB60 is a transcriptional
modulator of physiological responses in guard cells and open new possibilities to engineering
stomatal responses to improve plant survival during drought.
Tipologia IRIS:
03 - Contributo in volume
Keywords:
stomata ; guard cells ; plant water loss ; Arabidopsis thaliana
Elenco autori:
E. Cominelli, M. Galbiati, T. Sala, C. Tonelli
Link alla scheda completa:
Titolo del libro:
Proceedings of the 50th Italian Society of Agricultural Genetics Annual Congress