Layer- and subregion-specific electrophysiological and morphological changes of the medial prefrontal cortex in a mouse model of neuropathic pain
Articolo
Data di Pubblicazione:
2019
Citazione:
Layer- and subregion-specific electrophysiological and morphological changes of the medial prefrontal cortex in a mouse model of neuropathic pain / M. Mitrić, A. Seewald, G. Moschetti, P. Sacerdote, F. Ferraguti, K.K. Kummer, M. Kress. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 9(2019 Jul), pp. 9479.1-9479.13. [10.1038/s41598-019-45677-z]
Abstract:
Chronic neuropathic pain constitutes a serious public health problem, but the disease mechanisms are only partially understood. The involvement of different brain regions like the medial prefrontal cortex has already been established, but the comparison of the role of different subregions and layers is still inconclusive. In the current study, we performed patch-clamp recordings followed by anatomical reconstruction of pyramidal cells from different layers of the prelimbic and infralimbic subregions of the medial prefrontal cortex in neuropathic (spared nerve injury, SNI) and control mice. We found that in the prelimbic cortex, layer 2/3 pyramidal cells from SNI mice exhibited increased excitability compared to sham controls, whereas prelimbic layer 5 pyramidal neurons showed reduced excitability. Pyramidal cells in both layer 2/3 and layer 5 of the infralimbic subregion did not change their excitability, but layer 2/3 pyramidal cells displayed increased dendritic length and branching. Our findings support the view that chronic pain is associated with subregion- and layer-specific changes in the medial prefrontal cortex. They therefore provide new insights into the mechanisms underlying the chronification of pain.
Tipologia IRIS:
01 - Articolo su periodico
Elenco autori:
M. Mitrić, A. Seewald, G. Moschetti, P. Sacerdote, F. Ferraguti, K.K. Kummer, M. Kress
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