Data di Pubblicazione:
2019
Citazione:
Optimised Synthesis of the Bacterial Magic Spot (p)ppGpp Chemosensor PyDPA / G. Conti, M. Minneci, S. Sattin. - In: CHEMBIOCHEM. - ISSN 1439-4227. - 20:13(2019 Jul), pp. 1717-1721. [10.1002/cbic.201900013]
Abstract:
Guanosine penta- or tetraphosphate (pppGpp or ppGpp, respectively) is a nucleotide signalling molecule with a marked effect on bacterial physiology during stress. Its accumulation slows down cell metabolism and replication, supposedly leading to the formation of the antibiotic-tolerant persister phenotype. A specifically tailored fluorescent chemosensor, PyDPA, allows the detection of (p)ppGpp in solution with high selectivity, relative to that of other nucleotides. Herein, an optimised synthetic approach is presented that improves the overall yield from 9 to 67 % over 7 steps. The simplicity and robustness of this approach will allow groups investigating the many facets of (p)ppGpp easy access to this probe.
Tipologia IRIS:
01 - Articolo su periodico
Keywords:
fluorescence; guanosine phosphates; nucleotides; sensors; synthesis design
Elenco autori:
G. Conti, M. Minneci, S. Sattin
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